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dab staining  (Vector Laboratories)


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    Vector Laboratories dab staining
    Novel, modified peptides derived from netrin-1 exerted augmented effects in attenuating pulmonary vascular muscularization and cell proliferation in pulmonary hypertensive mice . Pulmonary hypertension was induced in mice by exposure to normobaric hypoxia (10% O 2 ) for three weeks. Osmotic pump was used to deliver modified peptides derived from netrin-1, V1P, V2P, V3P, V1S, V1T, V1D, V1C continuously. (A,D) Representative images and quantitative data of smooth muscle actin (SMA) expression by <t>immunohistochemical</t> <t>staining</t> using <t>DAB</t> substrate. (B,E) Representative images and quantitative data of proliferating cell nuclear antigen (PCNA) expression by immunohistochemical staining using DAB substrate. (C,F) Representative images and quantitative data of collagen deposition by Masson's Trichrome staining. Data indicate that pulmonary vascular muscularization, cell proliferation and fibrosis in hypoxia induced pulmonary hypertensive mice were completely attenuated by treatment with the modified netrin-1 peptides. Data are shown as Mean ± SEM. ∗∗∗p < 0.001 vs. Normoxia; ###p < 0.001 vs. Hypoxia. Normoxia (n = 5), Hypoxia (n = 5), Hypoxia + V1P (n = 5), Hypoxia + V2P (n = 5), Hypoxia + V3P (n = 5), Hypoxia + V1S (n = 5), Hypoxia + V1T (n = 5), Hypoxia + V1D (n = 5), Hypoxia + V1C (n = 5).
    Dab Staining, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dab staining/product/Vector Laboratories
    Average 96 stars, based on 1108 article reviews
    dab staining - by Bioz Stars, 2026-06
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    1) Product Images from "Novel and superior treatment of pulmonary hypertension with netrin-1 derived, modified and improved small peptides"

    Article Title: Novel and superior treatment of pulmonary hypertension with netrin-1 derived, modified and improved small peptides

    Journal: Redox Biology

    doi: 10.1016/j.redox.2025.103710

    Novel, modified peptides derived from netrin-1 exerted augmented effects in attenuating pulmonary vascular muscularization and cell proliferation in pulmonary hypertensive mice . Pulmonary hypertension was induced in mice by exposure to normobaric hypoxia (10% O 2 ) for three weeks. Osmotic pump was used to deliver modified peptides derived from netrin-1, V1P, V2P, V3P, V1S, V1T, V1D, V1C continuously. (A,D) Representative images and quantitative data of smooth muscle actin (SMA) expression by immunohistochemical staining using DAB substrate. (B,E) Representative images and quantitative data of proliferating cell nuclear antigen (PCNA) expression by immunohistochemical staining using DAB substrate. (C,F) Representative images and quantitative data of collagen deposition by Masson's Trichrome staining. Data indicate that pulmonary vascular muscularization, cell proliferation and fibrosis in hypoxia induced pulmonary hypertensive mice were completely attenuated by treatment with the modified netrin-1 peptides. Data are shown as Mean ± SEM. ∗∗∗p < 0.001 vs. Normoxia; ###p < 0.001 vs. Hypoxia. Normoxia (n = 5), Hypoxia (n = 5), Hypoxia + V1P (n = 5), Hypoxia + V2P (n = 5), Hypoxia + V3P (n = 5), Hypoxia + V1S (n = 5), Hypoxia + V1T (n = 5), Hypoxia + V1D (n = 5), Hypoxia + V1C (n = 5).
    Figure Legend Snippet: Novel, modified peptides derived from netrin-1 exerted augmented effects in attenuating pulmonary vascular muscularization and cell proliferation in pulmonary hypertensive mice . Pulmonary hypertension was induced in mice by exposure to normobaric hypoxia (10% O 2 ) for three weeks. Osmotic pump was used to deliver modified peptides derived from netrin-1, V1P, V2P, V3P, V1S, V1T, V1D, V1C continuously. (A,D) Representative images and quantitative data of smooth muscle actin (SMA) expression by immunohistochemical staining using DAB substrate. (B,E) Representative images and quantitative data of proliferating cell nuclear antigen (PCNA) expression by immunohistochemical staining using DAB substrate. (C,F) Representative images and quantitative data of collagen deposition by Masson's Trichrome staining. Data indicate that pulmonary vascular muscularization, cell proliferation and fibrosis in hypoxia induced pulmonary hypertensive mice were completely attenuated by treatment with the modified netrin-1 peptides. Data are shown as Mean ± SEM. ∗∗∗p < 0.001 vs. Normoxia; ###p < 0.001 vs. Hypoxia. Normoxia (n = 5), Hypoxia (n = 5), Hypoxia + V1P (n = 5), Hypoxia + V2P (n = 5), Hypoxia + V3P (n = 5), Hypoxia + V1S (n = 5), Hypoxia + V1T (n = 5), Hypoxia + V1D (n = 5), Hypoxia + V1C (n = 5).

    Techniques Used: Modification, Derivative Assay, Expressing, Immunohistochemical staining, Staining



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    Novel, modified peptides derived from netrin-1 exerted augmented effects in attenuating pulmonary vascular muscularization and cell proliferation in pulmonary hypertensive mice . Pulmonary hypertension was induced in mice by exposure to normobaric hypoxia (10% O 2 ) for three weeks. Osmotic pump was used to deliver modified peptides derived from netrin-1, V1P, V2P, V3P, V1S, V1T, V1D, V1C continuously. (A,D) Representative images and quantitative data of smooth muscle actin (SMA) expression by <t>immunohistochemical</t> <t>staining</t> using <t>DAB</t> substrate. (B,E) Representative images and quantitative data of proliferating cell nuclear antigen (PCNA) expression by immunohistochemical staining using DAB substrate. (C,F) Representative images and quantitative data of collagen deposition by Masson's Trichrome staining. Data indicate that pulmonary vascular muscularization, cell proliferation and fibrosis in hypoxia induced pulmonary hypertensive mice were completely attenuated by treatment with the modified netrin-1 peptides. Data are shown as Mean ± SEM. ∗∗∗p < 0.001 vs. Normoxia; ###p < 0.001 vs. Hypoxia. Normoxia (n = 5), Hypoxia (n = 5), Hypoxia + V1P (n = 5), Hypoxia + V2P (n = 5), Hypoxia + V3P (n = 5), Hypoxia + V1S (n = 5), Hypoxia + V1T (n = 5), Hypoxia + V1D (n = 5), Hypoxia + V1C (n = 5).
    Dab Staining, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Novel, modified peptides derived from netrin-1 exerted augmented effects in attenuating pulmonary vascular muscularization and cell proliferation in pulmonary hypertensive mice . Pulmonary hypertension was induced in mice by exposure to normobaric hypoxia (10% O 2 ) for three weeks. Osmotic pump was used to deliver modified peptides derived from netrin-1, V1P, V2P, V3P, V1S, V1T, V1D, V1C continuously. (A,D) Representative images and quantitative data of smooth muscle actin (SMA) expression by <t>immunohistochemical</t> <t>staining</t> using <t>DAB</t> substrate. (B,E) Representative images and quantitative data of proliferating cell nuclear antigen (PCNA) expression by immunohistochemical staining using DAB substrate. (C,F) Representative images and quantitative data of collagen deposition by Masson's Trichrome staining. Data indicate that pulmonary vascular muscularization, cell proliferation and fibrosis in hypoxia induced pulmonary hypertensive mice were completely attenuated by treatment with the modified netrin-1 peptides. Data are shown as Mean ± SEM. ∗∗∗p < 0.001 vs. Normoxia; ###p < 0.001 vs. Hypoxia. Normoxia (n = 5), Hypoxia (n = 5), Hypoxia + V1P (n = 5), Hypoxia + V2P (n = 5), Hypoxia + V3P (n = 5), Hypoxia + V1S (n = 5), Hypoxia + V1T (n = 5), Hypoxia + V1D (n = 5), Hypoxia + V1C (n = 5).
    Dab Staining Vectastain Elite Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Novel, modified peptides derived from netrin-1 exerted augmented effects in attenuating pulmonary vascular muscularization and cell proliferation in pulmonary hypertensive mice . Pulmonary hypertension was induced in mice by exposure to normobaric hypoxia (10% O 2 ) for three weeks. Osmotic pump was used to deliver modified peptides derived from netrin-1, V1P, V2P, V3P, V1S, V1T, V1D, V1C continuously. (A,D) Representative images and quantitative data of smooth muscle actin (SMA) expression by <t>immunohistochemical</t> <t>staining</t> using <t>DAB</t> substrate. (B,E) Representative images and quantitative data of proliferating cell nuclear antigen (PCNA) expression by immunohistochemical staining using DAB substrate. (C,F) Representative images and quantitative data of collagen deposition by Masson's Trichrome staining. Data indicate that pulmonary vascular muscularization, cell proliferation and fibrosis in hypoxia induced pulmonary hypertensive mice were completely attenuated by treatment with the modified netrin-1 peptides. Data are shown as Mean ± SEM. ∗∗∗p < 0.001 vs. Normoxia; ###p < 0.001 vs. Hypoxia. Normoxia (n = 5), Hypoxia (n = 5), Hypoxia + V1P (n = 5), Hypoxia + V2P (n = 5), Hypoxia + V3P (n = 5), Hypoxia + V1S (n = 5), Hypoxia + V1T (n = 5), Hypoxia + V1D (n = 5), Hypoxia + V1C (n = 5).
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    3 3 diaminobenzidine dab staining  (Vector Laboratories)
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    Novel, modified peptides derived from netrin-1 exerted augmented effects in attenuating pulmonary vascular muscularization and cell proliferation in pulmonary hypertensive mice . Pulmonary hypertension was induced in mice by exposure to normobaric hypoxia (10% O 2 ) for three weeks. Osmotic pump was used to deliver modified peptides derived from netrin-1, V1P, V2P, V3P, V1S, V1T, V1D, V1C continuously. (A,D) Representative images and quantitative data of smooth muscle actin (SMA) expression by <t>immunohistochemical</t> <t>staining</t> using <t>DAB</t> substrate. (B,E) Representative images and quantitative data of proliferating cell nuclear antigen (PCNA) expression by immunohistochemical staining using DAB substrate. (C,F) Representative images and quantitative data of collagen deposition by Masson's Trichrome staining. Data indicate that pulmonary vascular muscularization, cell proliferation and fibrosis in hypoxia induced pulmonary hypertensive mice were completely attenuated by treatment with the modified netrin-1 peptides. Data are shown as Mean ± SEM. ∗∗∗p < 0.001 vs. Normoxia; ###p < 0.001 vs. Hypoxia. Normoxia (n = 5), Hypoxia (n = 5), Hypoxia + V1P (n = 5), Hypoxia + V2P (n = 5), Hypoxia + V3P (n = 5), Hypoxia + V1S (n = 5), Hypoxia + V1T (n = 5), Hypoxia + V1D (n = 5), Hypoxia + V1C (n = 5).
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    Novel, modified peptides derived from netrin-1 exerted augmented effects in attenuating pulmonary vascular muscularization and cell proliferation in pulmonary hypertensive mice . Pulmonary hypertension was induced in mice by exposure to normobaric hypoxia (10% O 2 ) for three weeks. Osmotic pump was used to deliver modified peptides derived from netrin-1, V1P, V2P, V3P, V1S, V1T, V1D, V1C continuously. (A,D) Representative images and quantitative data of smooth muscle actin (SMA) expression by <t>immunohistochemical</t> <t>staining</t> using <t>DAB</t> substrate. (B,E) Representative images and quantitative data of proliferating cell nuclear antigen (PCNA) expression by immunohistochemical staining using DAB substrate. (C,F) Representative images and quantitative data of collagen deposition by Masson's Trichrome staining. Data indicate that pulmonary vascular muscularization, cell proliferation and fibrosis in hypoxia induced pulmonary hypertensive mice were completely attenuated by treatment with the modified netrin-1 peptides. Data are shown as Mean ± SEM. ∗∗∗p < 0.001 vs. Normoxia; ###p < 0.001 vs. Hypoxia. Normoxia (n = 5), Hypoxia (n = 5), Hypoxia + V1P (n = 5), Hypoxia + V2P (n = 5), Hypoxia + V3P (n = 5), Hypoxia + V1S (n = 5), Hypoxia + V1T (n = 5), Hypoxia + V1D (n = 5), Hypoxia + V1C (n = 5).
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    Novel, modified peptides derived from netrin-1 exerted augmented effects in attenuating pulmonary vascular muscularization and cell proliferation in pulmonary hypertensive mice . Pulmonary hypertension was induced in mice by exposure to normobaric hypoxia (10% O 2 ) for three weeks. Osmotic pump was used to deliver modified peptides derived from netrin-1, V1P, V2P, V3P, V1S, V1T, V1D, V1C continuously. (A,D) Representative images and quantitative data of smooth muscle actin (SMA) expression by immunohistochemical staining using DAB substrate. (B,E) Representative images and quantitative data of proliferating cell nuclear antigen (PCNA) expression by immunohistochemical staining using DAB substrate. (C,F) Representative images and quantitative data of collagen deposition by Masson's Trichrome staining. Data indicate that pulmonary vascular muscularization, cell proliferation and fibrosis in hypoxia induced pulmonary hypertensive mice were completely attenuated by treatment with the modified netrin-1 peptides. Data are shown as Mean ± SEM. ∗∗∗p < 0.001 vs. Normoxia; ###p < 0.001 vs. Hypoxia. Normoxia (n = 5), Hypoxia (n = 5), Hypoxia + V1P (n = 5), Hypoxia + V2P (n = 5), Hypoxia + V3P (n = 5), Hypoxia + V1S (n = 5), Hypoxia + V1T (n = 5), Hypoxia + V1D (n = 5), Hypoxia + V1C (n = 5).

    Journal: Redox Biology

    Article Title: Novel and superior treatment of pulmonary hypertension with netrin-1 derived, modified and improved small peptides

    doi: 10.1016/j.redox.2025.103710

    Figure Lengend Snippet: Novel, modified peptides derived from netrin-1 exerted augmented effects in attenuating pulmonary vascular muscularization and cell proliferation in pulmonary hypertensive mice . Pulmonary hypertension was induced in mice by exposure to normobaric hypoxia (10% O 2 ) for three weeks. Osmotic pump was used to deliver modified peptides derived from netrin-1, V1P, V2P, V3P, V1S, V1T, V1D, V1C continuously. (A,D) Representative images and quantitative data of smooth muscle actin (SMA) expression by immunohistochemical staining using DAB substrate. (B,E) Representative images and quantitative data of proliferating cell nuclear antigen (PCNA) expression by immunohistochemical staining using DAB substrate. (C,F) Representative images and quantitative data of collagen deposition by Masson's Trichrome staining. Data indicate that pulmonary vascular muscularization, cell proliferation and fibrosis in hypoxia induced pulmonary hypertensive mice were completely attenuated by treatment with the modified netrin-1 peptides. Data are shown as Mean ± SEM. ∗∗∗p < 0.001 vs. Normoxia; ###p < 0.001 vs. Hypoxia. Normoxia (n = 5), Hypoxia (n = 5), Hypoxia + V1P (n = 5), Hypoxia + V2P (n = 5), Hypoxia + V3P (n = 5), Hypoxia + V1S (n = 5), Hypoxia + V1T (n = 5), Hypoxia + V1D (n = 5), Hypoxia + V1C (n = 5).

    Article Snippet: For DAB staining, sections were incubated with secondary antibodies (1:200, Cat# PK-6102-mouse, NC9293436-rabbit, Vecta stain ABC kit, Vector labs, Burlingame, CA, USA) and stained using a 3,3-diaminobenzidine (DAB) substrate according to the manufacturer's instructions (MilliporeSigma, Cat# D3939, St. Louis, MO, USA).

    Techniques: Modification, Derivative Assay, Expressing, Immunohistochemical staining, Staining